high molecular weight hmw adiponectin  (ALPCO)

Journal: Antioxidants

Article Title: Therapeutic Potential of Cranberry Proanthocyanidins in Addressing the Pathophysiology of Metabolic Syndrome: A Scrutiny of Select Mechanisms of Action

doi: 10.3390/antiox14030268

Figure Lengend Snippet: Effect of PAC on insulin resistance, fasting lipid profile, lipoprotein composition, and inflammatory circulating biomarkers. At weeks 0, 6, and 10, ( A ) fasting glycemia and ( B ) fasting insulinemia was measured, enabling the calculation of the ( C ) Homeostatic model assessment of insulin resistance (HOMA-IR). At weeks 0, 6, 10, and 12, ( D ) fasting triglyceridemia and ( E ) fasting total cholesterolemia were measured. Results are presented as means ± SEM for n = 6–12 mice/group. At week 12, the lipid profile was further investigated to determine levels of ( F ) HDL-cholesterol and ( G ) non-HDL-cholesterol. Results are presented as means ± SEM for n = 4 pooled plasma/group. At week 12, plasma from n = 8 mice/group was collected and pooled for lipoprotein determination. Isolated fractions were first obtained via fast-protein liquid chromatography (FLPC) and then were analyzed to reveal content in ( H ) total cholesterol and ( I ) esterified cholesterol, as described in Materials and Methods. Circulating ( J ) adiponectin was measured at week 10. Circulating ( K ) interleukin-1-alpha (IL1A), ( L ) Monocyte Chemoattractant Protein-1 (MCP1), and ( M ) Tumor Necrosis Factor-alpha (TNFA) were obtained at week 12. Results are presented as means ± SEM for n = 6–8 mice/group. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. Chow; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. HFHF mice.

Article Snippet: Plasma samples ( n = 8 mice/group) obtained at week 10 were used to measure high molecular weight (HMW) adiponectin concentrations with the Mouse HMW and Total Adiponectin ELISA kit (ALPCO, Salem, NH, USA).

Techniques: Clinical Proteomics, Isolation, Fast Protein Liquid Chromatography

Journal: iScience

Article Title: Adipocyte Angptl8 deletion improves glucose and energy metabolism and obesity associated inflammation in mice

doi: 10.1016/j.isci.2024.111292

Figure Lengend Snippet: Construction and validation of adipose-specific conditional Angptl8 KO mice (A) Diagram of the Angptl8 gene with targeted ΔNeo allele, and the disrupted A8 allele. LoxP sites were placed upstream of exon 1 and downstream of exon 4. A vNEO cassette was inserted after exon 4. Mice with the targeted ΔNeo allele (Fl/Fl) were crossed with mice expressing Cre under the control of the adiponectin promoter to inactivate A8 in adipocytes (As-A8−/− mice). NDEL1 and NDEL2 primers used for genotyping are indicated. (B) The presence of the WT, Adipo-Cre transgene or the floxed A8 alleles was evaluated in DNA obtained from ear tissue biopsies using the primers as described in Methods. Specific amplification of a 686 bp DNA fragment corresponds to the floxed- A8 allele while a 569 bp DNA fragment corresponds to the WT A8 allele, the presence of a 600 bp DNA fragment denotes the Adipo-Cre transgene, as verified by genomic PCR. (C) Angptl8 mRNA expression in VAT, SAT, BAT and Liver tissue ( n = 4) were measured in 11-week-old male Cre and AT-A8-KO mice, 3 weeks after TMX treatment. Values are means ± SEM. Multiple unpaired t-test for multiple comparison were performed with Holm-Šídák correction using Graphpad Prism 10. (D) Angptl8 protein expression in collagenase treated mature adipocytes from VAT and SAT tissue in chow diet fed Cre and KO mice (n = 3–4); MM, Pre-stained Molecular weight markers. Quantification are shown in Figure S2 B. (E) Body weight ( n = 15), (F) lean mass and fat mass, ( n = 15) and (G) fasted glycemia ( n = 5) in chow diet fed 11-week-old Cre and AT-A8-KO mice. Data are means ± SEM. Groups were compared using unpaired t-test. For Cre vs. KO: ∗ p ≤ 0.033, ∗∗ p ≤ 0.002, ∗∗∗ p < 0.001. VAT, visceral adipose tissue; SAT, subcutaneous adipose tissue, BAT, brown adipose tissue.

Article Snippet: Mouse Total Adiponectin ELISA , ALPCO , Cat# 22-ADPMS-E01.

Techniques: Biomarker Discovery, Expressing, Control, Amplification, Comparison, Staining, Molecular Weight

Journal: iScience

Article Title: Adipocyte Angptl8 deletion improves glucose and energy metabolism and obesity associated inflammation in mice

doi: 10.1016/j.isci.2024.111292

Figure Lengend Snippet: AT-A8-KO Mice on HFHF diet Reduces Inflammatory Markers (A) MAC-2 immunostaining in VAT tissue section from Cre and KO mice on HFHF diet and (B) quantification of crown-like structures (CLS) ( n = 6 mice, 4 fields/tissue section, original magnification ×20; scale bar = 400 μm). Values are means ± SEM and groups were compared using unpaired t-test. ∗∗ p ≤ 0.002. (C) Plasma MCP-1, (D) Leptin and (E) Adiponectin levels in mice under fed state were quantified by ELISA or AlphaLISA (n = 8–10). Values are means ± SEM. Groups were compared using two-way ANOVA test using GraphPad Prism 10. ∗ p ≤ 0.033, ∗∗ p ≤ 0.002, ∗∗∗ p < 0.001.

Article Snippet: Mouse Total Adiponectin ELISA , ALPCO , Cat# 22-ADPMS-E01.

Techniques: Immunostaining, Clinical Proteomics, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Adipocyte Angptl8 deletion improves glucose and energy metabolism and obesity associated inflammation in mice

doi: 10.1016/j.isci.2024.111292

Figure Lengend Snippet:

Article Snippet: Mouse Total Adiponectin ELISA , ALPCO , Cat# 22-ADPMS-E01.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Activity Assay, Software